Cardiac’s drug safety: detection of acute and chronic effects

Cardiotoxicity is one of the leading causes of drug attrition. It has been one of the main reasons for drug withdrawals, accounting for 45% of all drugs taken off the market between 1994 and 2006.  However, cardiac drug safety is a major concern and one of the sticking points throughout cardiac drug development. Therefore, establishing in vitro assay at the early phases of drug development is critical in preventing late-stage failure.

In vitro cell’s culture are a powerful models for the accurate analysis of human heart disorders and therefore suitable to perform cardiotoxicity assays. Many of these disorders are the result of subtle changes to cardiomyocyte excitability, contractility, or both.

Our platform reveals the intracellular action potential you have been missing in fields such as cardiotoxicity and stem cell-derived cardiomyocyte differentiation, all label-free and in real-time. The cardiac action potential (AP), in fact, is vital for understanding and studied cardiac biology and drug safety .

Our tool demonstrated high reliability across different commercially available cardiomyocytes co-culture, with accurately measuring changes in depolarization, repolarization, and, indeed, detecting arrhythmias phenomena.

  • High quality and High spatial resolution: over minutes, hours or days, gain the access to single cell action potential

  • Massive action potential recording: thanks to an automated scanning motion, our platform enable also the recording from hundreds of cell action potential

  • Precise characterization of action potential: a noninvasively measurement of cardiac action potential morphology and easily detection of early afterdepolarizations (EADs). All these subtle alterations to action potential morphology like triangulation can be precisely detected. Action potential dynamic measured with our platform are comparable with manual patch clamp approach

  • Chronic and long-term detection of cardiotoxicity: a very low invasiveness assay allows chronic action potential recording from the same culture over time windows that can reach several days; It makes the platform useful for studying the “hidden cardiotoxicity”. The platform unlocks the possibility to predict precisely drug-related adverse affects that very often oblige their withdrawn from the market.

Stem-cell derived cardiac differentiation

Is it possible to capture the dynamic differentiation of stem cell-derived cardiomyocytes with just genetic expression data? Is it possible to track the emerging electrical activity of cardiac cells and follow the morphological changes ongoing in a syncytium that develops and matures?

Our system provides you all these information at the same time with only one experimental platform.

Thanks to a strong coupling with an optical path, our platform is able also to follow substantial changes in cell’s morphology, organization and structure that could arise after treatment with chemicals or molecules.

Exemplary cardiac action potentials on CMOS-MEA obtained with the FORESEE technology.
The signals are overlaid on the map showing the propagation of the cardiac activity over the 4096 electrodes of the MEA